Llama immunization and nanobody library construction
For nanobody generation, a recombinant murine LYVE1 protein, consisting of 288 amino acids, fused to a His10 tag, was expressed in human embryonic kidney 293 cells. The recombinant protein was…
Nanobody Immunolabelling and three-dimensional imaging reveals spatially restricted LYVE1 expression by kidney lymphatic vessels in mice | Journal of Nanobiotechnology
